Home > Protocols
DNA extraction protocol [DNA extraction.pdf]. We currently use Omega Biotek's kit and made a couple of slight modifications to their protocol.
cDNA Prep protocol [cDNA prep.pdf]. This is an adaptation of various protocols for extracting mRNA and for producing cDNA that can be used as a template in PCR or for other procedures. It is quick (15 minutes of work and 90 minutes of incubations) and easy and relatively inexpensive (and so we like it very much!). While we typically use this protocol with Conus venom ducts, the approach can be used with other tissues and other organisms (it was initially developed for producing cDNA from urchin gonads). This protocol was originally described by Duda & Palumbi (1999).
Cloning protocol [Cloning.pdf]. This is our modification of Invitrogen's TA cloning kit protocol. Our protocol utilizes lower volumes of reagents than recommended by Invitrogen which helps to substantially reduce cloning costs! The protocol is also written for new recruits to the clone wars.